biotinylated goat anti mouse slpi (R&D Systems)
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Biotinylated Goat Anti Mouse Slpi, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/biotinylated+goat+anti+mouse+slpi/Mouse+SLPI+Biotinylated+Antibody/pm35279880-68-53-57
Average 94 stars, based on 6 article reviews
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1) Product Images from "Secretory leukocyte protease inhibitor regulates nerve reflex-mediated skin barrier function in psoriasis."
Article Title: Secretory leukocyte protease inhibitor regulates nerve reflex-mediated skin barrier function in psoriasis.
Journal: Journal of the European Academy of Dermatology and Venereology : JEADV
doi: 10.1111/jdv.18065
Figure Legend Snippet: Figure 1 Secretory leukocyte protease inhibitor expression increases in lesional skin of psoriasis patients and in an experimental model of psoriasis. (a) Fluorescence microscopy images of human skin from the donors indicated. Overlay images from 2 healthy and 2 psoriatic donors are shown. Data are representative for at least 4 donors in each group. (b) Slpi gene expression changes during IMQ and Vaseline treatment in C57BL/6 mice. Control = non-treated mice. The data are shown as mean SD from three independent experiments. Dots indicate individual mice. *, P < 0.05; **, P < 0.01; *****, P < 0.00001; ******, P < 0.000001; by Kruskal–Wallis test with multiple com- parisons. (c) Representative images of SLPI protein expression in mouse skin during IMQ treatment. Skin sections were stained for SLPI (green) and DNA (blue). Data are from one experiment and are representative of at least five experiments. Image from SLPI KO mouse is shown as a negative control. Arrowheads in A & C point to SLPI-stained epidermis. Scale bar = 50 lm.
Techniques Used: Protease Inhibitor, Expressing, Fluorescence, Microscopy, Gene Expression, Control, Staining, Negative Control
Figure Legend Snippet: Figure 2 Secretory leukocyte protease inhibitor affects size of skin lesions and skin barrier function beyond the IMQ-challenged site. The mice indicated were subjected to IMQ-treatment followed by kinetic assessment of the lesion size and skin erythema. (a) The repre- sentative image of female WT and SLPI KO mice from mixed background C57BL/6 9 129/SvJ is shown with the indicated site of treat- ment. (b) Cumulative data (the mean SEM) showing size of lesion. n = 3–10 mice in each group. (c) Cumulative data (the mean SEM) showing skin erythema. (d) WT and SLPI KO C57BL6 males were subjected to IMQ-treatment followed by TEWL measure- ment at the days indicated at the central and lateral skin. The mean SEM is shown. n = at least 10 mice in each group. (e) WT and SLPI KO C57BL6 males were injected with 0.9% NaCl i.d. at the stimulated site prior to IMQ-treatment. TEWL was then measured at the days indicated at the central and lateral skin. The mean SEM is shown. n = at least 7 mice in each group. (f) The lateral skin of WT mice was harvested at the days indicated and subjected to qPCR analysis for Slpi mRNA levels. The data are shown as mean SD from three independent experiments. Dots indicate individual mice. *, P < 0.05; **, P < 0.01; ***, P < 0.001; by ANCOVA followed by Bonferroni post hoc test.
Techniques Used: Protease Inhibitor, Injection
Figure Legend Snippet: Figure 3 Secretory leukocyte protease inhibitor deficiency does not affect psoriatic skin thickness and has only a marginal effect on skin inflammation in experimental psoriasis. WT and SLPI KO C57BL/6 males were subjected to IMQ-treatment. (a) Skinfold thickness was measured at the days indicated using a caliper. The mean SEM is shown. n = 3–10 mice in each group. *****, P < 0.00001; **, P < 0.01; by two-way ANOVA followed by Bonferroni post hoc test. (b) Skin was harvested and analysed using histology. Results are rep- resentative of at least three independent experiments. (c) Skin was harvested and subjected to flow cytometry analysis. Total leukocytes were detected using anti CD45 mAbs, whereas neutrophils were detected using anti Ly6G and CD11b mAbs and T cells were detected using anti-CD3 mAbs. The mean of n = 7–10 mice SD is shown. *P < 0.05 by t-test.
Techniques Used: Protease Inhibitor, Cytometry
Figure Legend Snippet: Figure 5 A model of SLPI involvement in preventing excessive water loss in a reflex- arc regulated manner.
Techniques Used:
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